The ease of undertaking autoclave validation

Autoclave validation is a very important procedure that is mainly done for purposes of ensuring that the set requirements for the sterilizer’s usage have been met. Validation is an all important GMP (Good Manufacturing Practice) process that should be carried out regularly. Important variables that are used in a sterilizer’s validation include steam and temperature. A medical sterilizer is used for treating equipments and items used for medical purposes in hospitals. The variables (steam and temperature) are used for treating/ sterilizing these equipments. As such, there is need to ensure that a sterilizer is validate for it to be fully efficient.

How to validate temperature levels in an autoclave

The first thing to do when validating temperature in a sterilizer is that you should test the autoclave while recording the maximum temperatures reached. The sterilizer should be able to reach and maintain temperatures of 115, 121 & 132 Degrees Celsius. It is worth noting that high temperatures in autoclaves speed up the sterilization process thereby killing micro-organisms faster. You should attach an efficient data logger to a computer and the autoclave. The data logger records all important data details such as temperature as well as other physical parameters. You should read the data recorded on the logger to assess the efficiency of the autoclave. The data recorded on the logger should help you in making any necessary changes or adjustments on your autoclave.

How to validate steam

Steam validation helps in checking whether the sterilizer is able to supply steam that’s required for sterilization. When you want to validate steam, you should feed dry steam into the autoclave. Steam in a sterilizer is used in producing heat that’s in turn used in killing micro-organisms. You should use a biological indicator to test steam.

After feeding steam into the sterilizer, you should then prepare a paper (preferably a filter paper) that has bacterial spores. You should leave the spores in the autoclave for 24 hours at a 115 Degrees Celsius. After the relapse of the 24 hours, you should check any presence of live spores. Ideally, all the spores should have been killed by the steam, thereby indicating the efficiency of the process.

You should test the composition of the dry steam fed into the sterilizer. The steam should be comprised of 90% steam and 10% water. Not enough moisture in steam can lead to partiality in the sterilization process thereby making the process to be inefficient. For sterilization to be efficient and reliable, all the required physical conditions should be met. This brings about more accuracy and better efficiency in the whole sterilization process.

Autoclave validation needs to be done accurately for purposes of getting accurate results. In addition to this, there should also be the use of the right indicators for accuracy purposes. It is important to note that when the validation process is not done accurately, there’s a high possibility of getting wrong results. Such results do not depict the sterilizer’s actual working conditions. There is also need to ensure that the data recorded in the data logger is read and interpreted correctly.